CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly
Mathiowetz, A., Meymand, E., Deol, K.D., Parlakgul, G., Lange, M., Pange, S.P., Roberts, M.A., Torres, E., Boone, C., Zhang, Y., Morgens, D.W., Tso, E., Zhou, Y., Talukdar, S., Levine, T.P., Ku, G., Arruda, A.P.#, Olzmann, J.A.# (Under review)

Preprint: https://www.biorxiv.org/content/10.1101/2024.06.07.597858v1

Ferroptosis in Health and Disease
Berndt, C., ..., Olzmann, J.A., ..., Conrad, M.

Redox Biology. (2024) 30:75:103211.

Lipid droplets and cellular lipid flux

Mathiowetz, A. and Olzmann, J.A.

Nature Cell Biology. (2024) 26(3):331-345.

The cell biology of ferroptosis

Dixon, S.J.# and Olzmann, J.A.#

Nature Reviews Molecular Cell Biology. (2024) 25(6):424-442.

Selenium reduction of ubiquinone via SQOR suppresses ferroptosis

Lee, N., Park, S.J., Lange, M., Tseyang, T., Doshi, M., Kwon, T.Y., Song, Y., Greer, P.L., Olzmann, J.A., Spinelli, J., Kim, D.

Nature Metabolism. (2024) 6(2):343-358.

– Featured in News & Views by Chambers and Ratan (Nature Metabolism. 2024)

Preprint: https://www.biorxiv.org/content/10.1101/2023.04.13.535674v1

Small molecule correctors divert CFTR-F508del from ERAD by stabilizing sequential folding states

Riepe, C., Wachalska, M., Deol, K.K. Amaya, K. Porteus, M.H., Olzmann, J.A., Kopito, R.R.

Molecular Biology of the Cell. (2024) 35(2):ar15.

Preprint: https://www.biorxiv.org/content/10.1101/2023.09.15.556420v1

Lipid quality control and ferroptosis: From concept to mechanism

Li, Z.*#, Lange, M.*, Dixon, S.J., Olzmann, J.A.#

Annual Review of Biochemistry. (2023) Online ahead of print.

Sensitization of cancer cells to ferroptosis coincident with cell-cycle arrest

Rodencal, J., Kim, N., He, A., Li, V.L., Lange, M., He, J., Tarangelo, A., Schafer, Z.T., Olzmann, J.A., Long, J.Z., Sage, J., Dixon, S.J.

Cell Chemical Biology. (2023) 31, 1–15. Online ahead of print.

Preprint: https://www.biorxiv.org/content/10.1101/2023.07.19.549715v1

Parallel CRISPR-Cas9 screens reveal mechanisms of PLIN2 and lipid droplet regulation

Roberts, M.A., Deol, K.K., Lange, M., Leto, D., Mathiowetz, A.J., Stevenson, J., Hashemi, S.H., Morgens, D.W., Easter, E., Heydari, K., Nalls, M.A., Bassik, M.C., Kampmann, M., Kopito, R.R., Faghri, F., Olzmann, J.A.

Developmental Cell. (2023) 18:S1534-5807(23)00331-3.

Preprint: https://www.biorxiv.org/content/10.1101/2022.08.27.505556v1

Identification of structurally diverse FSP1 inhibitors that sensitize cancer cells to ferroptosis

Hendricks, J.M., Doubravsky, C.E., Wehri, E., Li, Z., Roberts, M.A., Deol, K.K., Lange, M., Lasheras-Otero, I., Momper, J.D., Dixon, S.J., Bersuker, K., Schlatetzky, J.#, Olzmann, J.A.#

Cell Chemical Biology. (2023) 30(9):1090-1103.e7.

Preprint: https://www.biorxiv.org/content/10.1101/2022.12.14.520445v1

Protocol for performing pooled CRISPR-Cas9 loss of function screens to identify genetic modifiers

Mathiowetz, A.*, Roberts, M.A.*, Morgens, D.W., Olzmann, J.A.#, Li, Z.#

STAR Protocols. (2023) 4(2):102201.

Hydropersulfides are endogenous antioxidants that inhibit ferroptosis

Lange, M. and Olzmann, J.A.

Cell Chemical Biology. (2022) 29(12):1661-1663.

Genome-wide CRISPR screens implicate plasma membrane asymmetry in exogenous ceramide toxicity

Morris, S.N.S., Deol, K.K., Lange, M., Olzmann, J.A. 

Biology Open. (2022) 11(12):bio059695.

Preprint: https://www.biorxiv.org/content/10.1101/2022.09.26.509629v1

VPS13A and VPS13C influence lipid droplet abundance

Chen, S.*, Roberts, M.A.*, Chen, C.Y., Markmiller, S., Wei, H.G., Yeo, G.W., Granneman, J.G., Olzmann, J.A.# Ferro-Novick, S.#

Contact. (2022) 5, 1-7.

Preprint: https://www.biorxiv.org/content/10.1101/2022.06.21.497109v1

Context-dependent regulation of ferroptosis sensitivity

Magtanong, L., Mueller, G.D., Williams, K.J., TKO Lab, Andrews, B., Boone, C., Moffat, J., Olzmann, J.A., Bensinger, S.J., Dixon, S.J.

Cell Chemical Biology. (2022) S2451-9456(22)00236-7.

– Featured in Preview by Lee and Gan (Cell Chem Biol. 2022)

End of the road: From the ER to the proteasome

Olzmann, J.A.

Nature Reviews Molecular Cell Biology. (2022) 23(8):520.

Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability

Li, Z., Ferguson, L., Deol, K.K., Roberts, M.A., Magtanong, L., Hendricks, J.M., Mousa, G.A., Kilinc, S., Schaefer, K., Wells, J.A., Bassik, M.C., Goga, A., Dixon, S.J., Ingolia, N., Olzmann, J.A. 

Nature Chemical Biology. (2022) 18(7):751-761.

Preprint: https://www.biorxiv.org/content/10.1101/2022.04.11.487892v1 – Featured in Research Watch (Cancer Discovery. 2022).

The Lipid Droplet Knowledge Portal: A resource for systematic analyses of lipid droplet biology

Mejhert N., Gabriel, K.R., Frendo-Cumbo, S., Krahmer, N., Song, J., Kuruvilla, L., Chitraju, C., Boland, S., Jang, D.K., von Grotthuss, M., Costanzo, M.C., Ryden, M., Olzmann, J.A., Flannick, J., Burtt, N.P., Farese, R.V., Walther, T.C.

Developmental Cell. (2022) 57, 387–397.

More Metabolism!

Olzmann, J.A., Fendt, S.M., Shah, Y., Vousden, K., Chandel, N., Horng, T., Danial, N., Tu, B., Christofk, H., Vander Heiden, M.G., Wellen, K.

Molecular Cell. (2021) 81(18):3659-3664.

Ending on a sour note: exogenous lipids orchestrate cancer cell death

Lange, M. and Olzmann, J.A. 

Cell Metabolism. (2021) 33(8):1507-1509.

Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells

Peterson, C.W.H.*, Deol, K.K.*, To, M., Olzmann, J.A.

STAR Protocols. (2021) 5;2(2):100579.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Klionsky, D., … Olzmann, J.A., et al.

Autophagy. (2021) 17(1):1-382.

Diversity through equity and inclusion: The responsibility belongs to all of us

Olzmann, J.A. 

Mol. Biol. Cell. (2020) 31(25):2757-2760.

Protein quality control and lipid droplet metabolism

Roberts, M.A. and Olzmann, J.A. 

Annu. Rev. Cell Dev. Biol. (2020) 36:115-139.

Organelle biogenesis: ER shape influences lipid droplet nucleation

Roberts, M.A., Segura-Roman, A., Olzmann, J.A. 

Current Biol. (2020) 30(13), R770–R773.

A genome-wide screen for ER autophagy highlights key roles of mitochondrial metabolism and ER-resident UFMylation

Liang, J.R.*, Lingeman, E.*, Luong, T., Ahmed, S., Nguyen, T., Olzmann, J.A., Corn, J.  

Cell. (2020) 180, 1–18. PMID: 32160526.

– Preprint: https://www.biorxiv.org/content/10.1101/561001v1

– Featured in Research Highlight by Paulina Strzyz (Nat. Rev. Mol. Cell Biol. 2020)

– Featured in Spotlight by Maurizio Molinari (Mol. Cell. 2020)

Going through a phase

Li, Z., Morris, S.N.S., Olzmann, J.A.

Nature Chemical Biology. (2020) 16(2):111-112.

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis

Bersuker, K., Hendricks, J., Li, Z., Magtanong, L., Ford, B., Tang, P.H., Roberts, M.A., Tong, B., Maimone, T.J., Zoncu, R., Nomura, D.K., Bassik, M.C., Dixon, S.J., Olzmann, J.A. 

Nature. (2019) 575(7784):688-692.

– Featured in News & Views by Brent Stockwell (Nature. 2019)

– Featured in Research Highlight by Grant Miura (Nat. Chem. Biol. 2019)

– Featured in Research Highlight by Liang et al. (Cell Res. 2019)

– Featured in Preview by Massimo Santoro (Cell Metab. 2020)

– Featured in Viewpoint by Kamyar Hadian (Biochemistry 2020).

Covalent targeting of the vacuolar H+-ATPase enhances cellular clearance through inhibition of lysosomal mTORC1 signaling

Chung C.Y.S., Shin, H.R., Berdan, C.A., Ford, B., Ward, C.C., Olzmann J.A., Zoncu, R., Nomura, D.K.

Nature Chemical Biology. (2019) 15(8):776-785.

 A tense situation: Maintaining ER homeostasis during lipid droplet budding

Morris, S.N.S. and Olzmann, J.A.

Developmental Cell. (2019) 50, 1-2.

Harnessing the anti-cancer natural product nimbolide for targeted protein degradation

Spradlin, J.N., Hu, X., Ward, C.C., Brittain, S.M., Jones, M.D., Ou, L., To, M., Proudfoot, A., Ornelas, E., Woldegiorgis, M., Olzmann, J.A., Bussiere, D.E., Thomas, J.R., Tallarico, J.A., McKenna, J.M., Schirle, M., Maimone, T.J., Nomura, D.K.

Nature Chemical Biology. (2019) 15(7):747-755. 

– Preprint: https://www.biorxiv.org/content/10.1101/436998v2

– Featured in News & Views by Brand and Winter (Nat. Chem. Biol. 2019)

Identification of lipid droplet proteomes by proximity labeling proteomics using APEX2

Bersuker, K. and Olzmann, J.A.

Methods Molecular Biology - Proximity Labeling. (2019) 57-72.

Parthenolide impairs breast cancer pathogenicity through targeting an allosteric cysteine on focal adhesion kinase

Berdan, C.A., Ho, R., Lehtola, H.S., To, M., Hu, X., Huffman, T.R., Petri, Y., Altobelli, C.R., Demeulenaere, S.G., Olzmann, J.A., Maimone, T.J., Nomura, D.K.

Cell Chemical Biology. (2019) 26(7):1027-1035. PMID: 31080076.

– Preprint: https://www.biorxiv.org/content/10.1101/550806v2

Getting a handle on lipid droplets: Insights into ER-lipid droplet tethering

Nguyen, T. and Olzmann J.A.

Journal of Cell Biology. (2019) 218(4):1089-1091.

Exogenous monounsaturated fatty acids suppress non-apoptotic cell death

Magtanong, L., Ko, P.J., To, M., Cao, J.Y., Tarangelo, A.N., Ward, C., Nomura, D.K., Olzmann, J.A., Dixon, S.

Cell Chemical Biology. (2019) 26, 420–432.

–  Featured in Preview by Unduri Das (Cell Chem. Biol., 26, 309-311)

Dynamics and functions of lipid droplets

Olzmann, J.A.# and Carvalho, P.#

Nature Reviews Molecular Cell Biology. (2019) 20(3): 137-155.

A proteomic map to navigate subcellular reorganization in fatty liver disease

Li, Z. and Olzmann, J.A.

Developmental Cell. (2018) 47, 139-141.

In close proximity: The lipid droplet proteome and crosstalk with the ER

Bersuker, K. and Olzmann, J.A. 
Contact. (2018) (1), 1-3.

A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates

Huang, E.Y.*, To, M.*, Tran, E., Dionisio, L., Cho, H.J., Baney, K.L.M., Pataki, C.I., Olzmann, J.A.

Molecular Biology of the Cell. (2018) 29(9), 1021-1030.

– Featured as an MBoC highlight.

– Featured on the cover of the journal.

A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteomes

Bersuker, K., Peterson, C.W., To, M., Sahl, S.J., Savikhin, V., Grossman, E.A., Nomura, D.K., Olzmann, J.A. 

Developmental Cell. (2018) 44, 97-112.

–  Featured in Preview by Joel Goodman (Dev. Cell, 44, 1-2)

Chemoproteomics-Enabled Covalent Ligand Screening Reveals a Thioredoxin-Caspase 3 Interaction Disruptor That Impairs Breast Cancer Pathogenicity

Anderson, K.E., To, M., Olzmann, J.A., Nomura, D.K.

ACS Chemical Biology. (2017) 12(10), 2522-2528.

Lipid droplets and lipotoxicity during autophagy

Nguyen, T.B. and Olzmann, J.A. 

Autophagy. (2017) 13:11, 2002-2003.

DGAT1-dependent lipid droplet biogenesis protects mitochondrial function during starvation-induced autophagy

Nguyen, T.B., Louie, S.M., Daniele, J., Tran, Q., Dillin, A., Zoncu, R., Nomura, D.K., Olzmann, J.A. 

Developmental Cell. (2017) 42, 9–21.

–  Featured in Preview by Klecker et al. (Dev. Cell 41, 1-2)

–  Featured in Comment by Li et al. (Autophagy 13, 1995-1997)

–  Featured in Best of Dev. Cell 2017

Establishing the lipid droplet proteome: Mechanisms of lipid droplet protein targeting and degradation

Bersuker, K. and Olzmann, J.A. 

BBA - Molecular and Cell Biology of Lipids. (2017) 1862(10 Pt B), 1166-1177.

Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1

Hwang, J., Walczak, C.P., Shaler, T.A., Olzmann, J.A., Zhang, L., Elias, J.E., Kopito, R.R.

Journal of Biological Chemistry. (2017) 292, 9104-9116.

Chemoproteomics-enabled covalent ligand screen reveals a cysteine hotspot in Reticulon 4 that impairs ER morphology and cancer pathogenicity 

Bateman, L.A., Nguyen, T.B., Roberts, A.M., Miyamoto, D.K., Ku, W.M., Huffman, T.R., Petri, Y., Heslin, M.J., Contreras, C.M., Skibola, C.F., Olzmann, J.A.#, Nomura, D.K.#

Chemical Communications. (2017) 53(53), 7234-7237.

Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation

To, M.*, Peterson, C.W.*, Roberts, M.A., Counihan, J.L., Wu, T.T., Forster, M.S., Nomura, D.K., Olzmann, J.A.

Molecular Biology of the Cell. (2017) (28), 270-284.

Endoplasmic reticulum-associated degradation and lipid homeostasis

Stevenson, J.*, Huang, E.Y.*, Olzmann, J.A.

Annual Review of Nutrition. (2016) 36, 17.1–17.32.

A polyubiquitin chain reaction: Parkin recruitment to damaged mitochondria

Riley, B.E., Olzmann, J.A. 

PLOS Genet. (2015) 11(1), e1004952.

Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover

Olzmann, J.A., Richter, C.R., Kopito, R.R.

Proc. Natl. Acad. Sci. (2013) 110(4), 1345-50.

The mammalian endoplasmic reticulum-associated degradation system 

Olzmann, J.A., Kopito, R.R., Christianson, J.C.

Cold Spring Harb. Perspect. Biol. (2013) cshperspect.a013185v1. 1;5(9), pii: a013185.

– Featured on the cover of the journal.

Unassembled CD147 is an endogenous ER-associated degradation (ERAD) substrate 

Tyler, R.E., Pearce, M.M.P., Shaler, T.A., Olzmann, J.A., Greenblatt, E.J., Kopito, R.R.

Mol. Biol. Cell. (2012) 23(24), 4668-78.

–  Faculty of 1000 recommended.

Making the cut: Intramembrane cleavage by a rhomboid protease promotes ERAD

Greenblatt, E.J., Olzmann, J.A., Kopito, R.R.

Nature Struct. Mol. Biol. (2012) 19(10), 979-981.

–  Faculty of 1000 recommended.

Defining human ERAD networks through an integrated mapping strategy

Christianson, J.C.*, Olzmann, J.A.*, Shaler, T.A., Sowa, M.E., Bennett, E.J., Richter, C.M., Tyler, R.E., Greenblatt, E.J., Harper, J.W., Kopito, R.R.

* These authors contributed equally.

Nature Cell Biol. (2012) 14(1), 93-105.

–  Featured in News and Views by Thibault Mayor (Nat Cell Biol 14(1), 46-47)

–  Faculty of 1000 recommended.

Derlin-1 is a rhomboid protein required for dislocation of mutant a-1-antitrypsin from the endoplasmic reticulum

Greenblatt, E.J., Olzmann, J.A., Kopito, R.R.

Nature Struct. Mol. Biol. (2011) 18(10), 1147-1152.

Mutations associated with Charcot-Marie-Tooth disease cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways

Lee, S.M., Olzmann, J.A., Chin, L.S., Li, L.

J. Cell Sci. (2011) 124, 3319-3331.

Lipid droplets are dispensable for endoplasmic reticulum-associated degradation

Olzmann, J.A., Kopito, R.R.

J. Biol. Chem. (2011) 286, 27872-27874.

– Faculty of 1000 recommended.

Parkin-mediated ubiquitin signaling in aggresome formation and autophagy

Chin, L.S., Olzmann, J.A., Li, L.

Biochem. Soc. Trans. (2010) 48(1), 144-149.

SPFH1 and SPFH2 mediate the ubiquitination and degradation of inositol 1,4,5-trisphosphate receptors in muscarinic receptor-expressing HeLa cells

Wang, Y., Pearce, M.M., Sliter, D.A., Olzmann, J.A., Christianson, J.C., Kopito, R.R., Boeckmann, S., Gagen, C., Leichner, G.S., Roitelman, J., Wojcikiewicz, R.J.

Biochim. Biophys. Acta. (2009) 1793(11), 1710-8.

Corneal dystrophy-associated R124H mutation disrupts TGFBI interaction with Periostin and causes mislocalization to the lysosome

Kim, B.Y., Olzmann, J.A., Choi, S.I., Ahn, S.Y., Kim, T.I., Cho, H.S., Suh, H., Kim, E.K.

J. Biol. Chem. (2009) 284,19580-19591.

Aggresome formation and neurodegenerative diseases: therapeutic implications

Olzmann, J.A., Li, L., Chin, L.S.

Curr. Med. Chem. (2008) 15(1), 47-60.

Parkin-mediated K63-linked polyubiquitination: a signal for targeting misfolded proteins to the aggresome-autophagy pathway

Olzmann, J.A., Chin, L.S.

Autophagy. (2008) 4(1), 85-87.

Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding HDAC6

Olzmann, J.A., Li, L., Chudaev, M., Perez, F.A., Palmiter, R. D., Chin, L.S.

J. Cell Biol. (2007) 178, 1025-1038.

PINK1 protects against oxidative stress-induced apoptosis by phosphorylating mitochondrial chaperone TRAP1/Hsp75

Pridgeon, J.W.*, Olzmann, J.A.*, Chin, L.S., Li, L.

* These authors contributed equally.

PLOS Biol. (2007) 5, e172. PMID: 17579517.

–  Featured in News and Views by Asa Abeliovich (Nature 448, 759-760)

–  Primer by Kroemer and Blomgren (PLoS Biol 5(7): e206)

–  Faculty of 1000 recommended.

Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking

Kim, B.Y.*, Olzmann, J.A.*, Barsh, G.S., Chin, L.S., Li, L.

* These authors contributed equally.

Mol. Biol. Cell. (2007) 18, 1129-1142. PMID: 17229889.

–  Faculty of 1000 recommended.

Selective enrichment of DJ-1 protein in primate striatal neuronal processes: Implications for Parkinson’s disease

Olzmann, J.A., Bordelon, J.R., Muly, E.C., Rees, H.D., Levey, A.I., Li, L, Chin, L.S.

J. Comp. Neurol. (2007) 500, 585-599.

Oxidative damage of DJ-1 is linked to sporadic Parkinson's and Alzheimer's diseases

Choi, J., Sullards, C.M., Olzmann, J.A., Rees, H.D., Weintraub, S.T., Bostwick, D.E., Gearing, M., Levey, A.I., Chin, L.S., Li, L.

J. Biol. Chem. (2006) 281, 10816-24.

Uncoupling proteins prevent glucose-induced neuronal oxidative stress and programmed cell death

Vincent, A.M., Olzmann, J.A., Brownlee, M., Sivitz, W.I., and Russell, J.W.

Diabetes. (2004) 53, 726-734.

Familial Parkinson's disease-associated L166P mutation disrupts DJ-1 protein folding and function

Olzmann, J. A., Brown, K., Wilkinson, K. D., Rees, H. D., Huai, Q., Ke, H., Levey, A. I., Li, L., and Chin, L. S.

J. Biol. Chem. (2004) 279, 8506-8515.

High glucose-induced oxidative stress and mitochondrial dysfunction in neurons 

Russell, J.W., Golovoy, D., Vincent, A.M., Mahendru, P., Olzmann, J.A., Mentzer, A., and Feldman, E.L.

FASEB J. (2002) 16, 1738-1748.