Parallel CRISPR-Cas9 screens reveal mechanisms of PLIN2 and lipid droplet regulation

Roberts, M.A., Deol, K.K., Lange, M., Leto, D., Mathiowetz, A.J., Stevenson, J., Hashemi, S.H., Morgens, D.W., Easter, E., Heydari, K., Nalls, M.A., Bassik, M.C., Kampmann, M., Kopito, R.R., Faghri, F., Olzmann, J.A. (Submitted)

bioRxiv link posted 08.29.2022

Identification of structurally diverse FSP1 inhibitors that sensitize cancer cells to ferroptosis

Hendricks, J., Wehri, E., Doubravsky, C. Li, Z., Roberts, M.A., Deol, K.K., Lange, M., Dixon, S.J., Bersuker, K., Schlatetzky, J.#, Olzmann, J.A.# (Submitted)

bioRxiv link posted 12.14.2022

Hydropersulfides are endogenous antioxidants that inhibit ferroptosis

Lange, M. and Olzmann, J.A.

Cell Chemical Biology. (2022) 29(12):1661-1663. 

A genome-wide CRISPR screen implicates plasma membrane asymmetry in exogenous C6-ceramide toxicity

Morris, S.N.S., Deol, K.K., Lange, M., Olzmann, J.A.

Biology Open. (2022) Online ahead of print.

bioRxiv link posted 09.28.2022

 VPS13A and VPS13C influence lipid droplet abundance

Chen, S.*, Roberts, M.A.*, Chen, C.Y., Markmiller, S., Wei, H.G., Yeo, G.W., Granneman, J.G., Olzmann, J.A., Ferro-Novick, S.#

Contact. (2022) 5, 1-7.

bioRxiv link posted 06.23.2022

Context-dependent regulation of ferroptosis sensitivity

Magtanong, L., Mueller, G.D., Williams, K.J., TKO Lab, Andrews, B., Boone, C., Moffat, J., Olzmann, J.A., Bensinger, S.J., Dixon, S.J.

Cell Chemical Biology (2022) S2451-9456(22)00236-7.

End of the road: From the ER to the proteasome

Olzmann, J.A.

Nature Review of Molecular and Cell Biology (2022) 23(8):520.

Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability

Li., Z., Ferguson, L., Deol, K.K., Roberts, M.A., Magtanong, L., Hendricks, J.M., Mousa, G.A., Kilinc, S., Schaefer, K., Wells, J.A., Bassik, M.C., Goga, A., Dixon, S.J., Ingolia, N., Olzmann, J.A.

Nature Chemical Biology (2022) 18(7):751-761.

bioRxiv link posted 04.11.2022

  – Featured in Research Watch (Cancer Discovery. 2022)

The Lipid Droplet Knowledge Portal: A resource for systematic analyses of lipid droplet biology

Mejhert N., Gabriel, K.R., Frendo-Cumbo, S., Krahmer, N., Song, J., Kuruvilla, L., Chitraju, C., Boland, S., Jang, D.K., von Grotthuss, M., Costanzo, M.C., Ryden, M., Olzmann, J.A., Flannick, J., Burtt, N.P., Farese, R.V., Walther, T.C.

Developmental Cell. (2022) 57, 387–397.

More Metabolism!

Olzmann, J.A., Fendt, S.M., Shah, Y., Vousden, K., Chandel, N., Horng, T., Danial, N., Tu, B., Christofk, H., Vander Heiden, M.G., Wellen, K.

Molecular Cell. (2021) 81(18):3659-3664.

Ending on a sour note: Lipids orchestrate ferroptosis in cancer

Lange, M., Olzmann, J.A.

Cell Metabolism. (2021) 33(8):1507-1509.

Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells

Peterson, C.W.H.*, Deol, K.K.*, To, M., Olzmann, J.A.

STAR Protocols. (2021) 5;2(2):100579.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Klionsky, D. ... Olzmann, J.A., et al. 

Autophagy. (2021) 17(1):1-382.

Diversity through equity and inclusion: The responsibility belongs to all of us.

Olzmann, J.A.

Molecular Biology of the Cell. (2020) 31(25):2757-2760.

Protein quality control and lipid droplet metabolism.

Roberts, M.A., Olzmann, J.A.

Annual Review of Cell Developmental Biology. (2020) 36:115-139.

Organelle biogenesis: ER shape influences lipid droplet nucleation.

Roberts, M.A., Segura-Roman, A., Olzmann, J.A.

Current Biology. (2020) 30(13), R770–R773.

A genome-wide screen for ER autophagy highlights key roles of mitochondrial metabolism and ER-resident UFMylation.

Liang, J.R.*, Lingeman, E.*, Luong, T., Ahmed, S., Nguyen, T., Olzmann, J.A., Corn, J.

Cell. (2020) 180, 1–18.

  – Featured in Research Highlight by Paulina Strzyz (Nat. Rev. Mol. Cell Biol. 2020)

  – Featured in Spotlight by Maurizio Molinari (Mol. Cell. 2020)

Going through a phase.

Li, Z., Morris, S.N.S., Olzmann, J.A.

Nature Chemical Biology. (2020) 16(2):111-112.

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Bersuker, K., Hendricks, J., Li, Z., Magtanong, L., Ford, B., Tang, P.H., Roberts, M.A., Tong, B., Maimone, T.J., Zoncu, R., Nomura, D.K., Bassik, M.C., Dixon, S.J., Olzmann, J.A.

Nature. 575(7784):688-692.(2019) 
  – Featured in News & Views by Brent Stockwell (Nature 2019)

  – Featured in Research Highlight by Grant Miura (Nat. Chem. Biol. 2019)

  – Featured in Research Highlight by Liang et al. (Cell Res. 2019)

  – Featured in Preview by Massimo Santoro (Cell Metab. 2020)

  – Featured in Viewpoint by Kamyar Hadian (Biochemistry 2020)

Covalent targeting of the vacuolar H+-ATPase enhances cellular clearance through inhibition of lysosomal mTORC1 signaling.

Chung C.Y.S., Shin, H.R., Berdan, C.A., Ford, B., Ward, C.C., Olzmann J.A., Zoncu, R., Nomura, D.K.

Nature Chemical Biology. (2019) 15(8):776-785.

A tense situation: Maintaining ER homeostasis during lipid droplet budding.

Morris, S.N.S., Olzmann, J.A.

Developmental Cell. (2019) 50, 1-2.

Harnessing the anti-cancer natural product nimbolide for targeted protein degradation.

Spradlin, J.N., Hu, X., Ward, C.C., Brittain, S.M., Jones, M.D., Ou, L., To, M., Proudfoot, A., Ornelas, E., Woldegiorgis, M., Olzmann, J.A., Bussiere, D.E., Thomas, J.R., Tallarico, J.A., McKenna, J.M., Schirle, M., Maimone, T.J., Nomura, D.K.

Nature Chemical Biology. (2019) 15(7):747-755.

  – Featured in News & Views by Brand and Winter (Nat. Chem. Biol. 2019)

Identification of lipid droplet proteomes by proximity labeling proteomics using APEX2.

Bersuker, K., Olzmann, J.A.

Methods Molecular Biology. - Proximity Labeling. (2019) 57-72.

Parthenolide impairs breast cancer pathogenicity through targeting an allosteric cysteine on focal adhesion kinase.

Berdan, C.A., Ho, R., Lehtola, H.S., To, M., Hu, X., Huffman, T.R., Petri, Y., Altobelli, C.R., Demeulenaere, S.G., Olzmann, J.A., Maimone, T.J., Nomura, D.K.

Cell Chemical Biology. (2019) 26(7):1027-1035.

Getting a handle on lipid droplets: Insights into ER-lipid droplet tethering.

Nguyen, T., Olzmann J.A. 

Journal of Cell Biology. (2019) 218(4):1089-1091.

Exogenous monounsaturated fatty acids suppress non-apoptotic cell death.

Magtanong, L., Ko, P.J., To, M., Cao, J.Y., Tarangelo, A.N., Ward, C., Nomura, D.K., Olzmann, J.A., Dixon, S.

Cell Chemical Biology. (2019) 26, 420–432.

  –  Featured in Preview by Undurti Das (Cell Chem. Biol., 26, 309-311)

Dynamics and functions of lipid droplets.

Olzmann, J.A., Carvalho, P.

Nature Review of Molecular and Cell Biology. (2019) 20(3): 137-155.

A proteomic map to navigate subcellular reorganization in fatty liver disease.

Li, Z., Olzmann, J.A.

Developmental Cell. (2018) 47, 139-141.

In close proximity: The lipid droplet proteome and crosstalk with the ER.

Bersuker, K, Olzmann, J.A.

Contact. (2018) (1), 1-3.

A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates.

Huang, E.Y.*, To, M.*, Tran, E., Dionisio, L., Cho, H.J., Baney, K.L.M., Pataki, C.I., Olzmann, J.A.

Molecular Biology of the Cell. (2018) 29(9), 1021-1030.

A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteomes.

Bersuker, K., Peterson, C.W., To, M., Sahl, S.J., Savikhin, V., Grossman, E.A., Nomura, D.K., Olzmann, J.A.

Developmental Cell. (2018) 44, 97-112.

  –  Featured in Preview by Joel Goodman (Dev. Cell, 44, 1-2)

Chemoproteomics-Enabled Covalent Ligand Screening Reveals a Thioredoxin-Caspase 3 Interaction Disruptor That Impairs Breast Cancer Pathogenicity.

Anderson, K.E., To, M., Olzmann, J.A., Nomura, D.K.

ACS Chemical Biology. (2017) 12(10), 2522-2528.

Lipid droplets and lipotoxicity during autophagy.

Nguyen, T.B., Olzmann, J.A.

Autophagy. (2017) 13:11, 2002-2003.

DGAT1-dependent lipid droplet biogenesis protects mitochondrial function during starvation-induced autophagy.

Nguyen, T.B., Louie, S.M., Daniele, J., Tran, Q., Dillin, A., Zoncu, R., Nomura, D.K., Olzmann, J.A.

Developmental Cell. (2017) 42, 9–21.

  –  Featured in Preview by Klecker et al. (Dev. Cell 41, 1-2)

  –  Featured in Comment by Li et al. (Autophagy 13, 1995-1997)

  –  Featured in Best of Dev. Cell 2017

Establishing the lipid droplet proteome: Mechanisms of lipid droplet protein targeting and degradation.

Bersuker, K., Olzmann, J.A.

BBA - Molecular and Cell Biology of Lipids. (2017) 1862(10 Pt B), 1166-1177.

Characterization of protein complexes of the endoplasmic reticulum associated degradation E3 ubiquitin ligase Hrd1.

Hwang, J., Walczak, C.P., Shaler, T.A., Olzmann, J.A., Zhang, L., Elias, J.E., Kopito, R.R.

Journal of Biological Chemistry. (2017) 292, 9104-9116.

Chemoproteomics-enabled covalent ligand screen reveals a cysteine hotspot in Reticulon 4 that impairs ER morphology and cancer pathogenicity.

Bateman, L.A.*, Nguyen, T.B.*, Roberts, A.M.*, Miyamoto, D.K., Ku, W.M., Huffman, T.R., Petri, Y., Heslin, M.J., Contreras, C.M., Skibola, C.F., Olzmann, J.A.#, Nomura, D.K.#

# indicates co-corresponding authors

Chemical Communications. (2017) Jun 29;53(53), 7234-7237.

Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation.

To, M.*, Peterson, C.W.*, Roberts, M.A., Counihan, J.L., Wu, T.T., Forster, M.S., Nomura, D.K., Olzmann, J.A.​

Molecular Biology of the Cell. (2017) (28), 270-284.

Endoplasmic reticulum-associated degradation and lipid homeostasis.

Stevenson, J.*, Huang, E.Y.*, Olzmann, J.A.

Annual Review of Nutrition. (2016) 36, 17.1–17.32.

A polyubiquitin chain reaction: Parkin recruitment to damaged mitochondria.

Riley, B.E., Olzmann, J.A.

PLOS Genet. (2015) 11(1), e1004952.

Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.

Olzmann, J.A., Richter, C.R., Kopito, R.R.

Proc. Natl. Acad. Sci. (2013) 110(4), 1345-50.

The mammalian endoplasmic reticulum-associated degradation system.

Olzmann, J.A., Kopito, R.R., Christianson, J.C.

Cold Spring Harb. Perspect. Biol. (2013) cshperspect.a013185v1. 1;5(9), pii: a013185.

 

Unassembled CD147 is an endogenous ER-associated degradation (ERAD) substrate.

Tyler, R.E., Pearce, M.M.P., Shaler, T.A., Olzmann, J.A., Greenblatt, E.J., Kopito, R.R.

Molecular Biology of the Cell. (2012) 23(24), 4668-78.

Making the cut: Intramembrane cleavage by a rhomboid protease promotes ERAD.

Greenblatt, E.J., Olzmann, J.A., Kopito, R.R.

Nature Structural and Molecular Biology. (2012) 19(10), 979-981.

Defining human ERAD networks through an integrated mapping strategy.

Christianson, J.C.*, Olzmann, J.A.*, Shaler, T.A., Sowa, M.E., Bennett, E.J., Richter, C.M., Tyler, R.E., Greenblatt, E.J., Harper, J.W., Kopito, R.R.

* These authors contributed equally.

Nature Cell Biology. (2012) 14(1), 93-105.

  –  Featured in News and Views by Thibault Mayor (Nat Cell Biol 14(1), 46-47)

Derlin-1 is a rhomboid protein required for dislocation of mutant a-1-antitrypsin from the endoplasmic reticulum.

Greenblatt, E.J., Olzmann, J.A., Kopito, R.R.

Nature Structural and Molecular Biology. (2011) 18(10), 1147-1152.

Charcot-Marie-Tooth disease-associated mutations cause SIMPLE protein mislocalization and degradation by the proteasome and aggresome-autophagy pathways.

Lee, S.M., Olzmann, J.A., Chin, L.S., Li, L.

Journal of Cell Science. (2011) 124, 3319-3331.

Lipid droplets are dispensable for endoplasmic reticulum-associated degradation.

Kopito, R.R., Olzmann, J.A.

Journal of Biological Chemistry. (2011) 286, 27872-27874.

Parkin-mediated ubiquitin signaling in aggresome formation and autophagy.

Chin, L.S., Olzmann, J.A., Li, L.

Biochem. Soc. Trans. (2010) 48(1), 144-149.

An ER membrane complex composed of SPFH1 and SPFH2 mediates the ER-associated degradation of IP3 receptors.

Wang, Y., Pearce, M.M., Sliter, D.A., Olzmann, J.A., Christianson, J.C., Kopito, R.R., Boeckmann, S., Gagen, C., Leichner, G.S., Roitelman, J., Wojcikiewicz, R.J.

Biochim. Biophys. Acta. (2009) 1793(11), 1710-8.

Corneal dystrophy-associated R124H mutation disrupts TGFBI interaction with Periostin and causes mislocalization to the lysosome.

Kim, B.Y., Olzmann, J.A., Choi, S.I., Ahn, S.Y., Kim, T.I., Cho, H.S., Suh, H., Kim, E.K.

Journal of Biological Chemistry. (2009) 284,19580-19591.

Aggresome formation and neurodegenerative diseases: therapeutic implications.

Olzmann, J.A., Li, L., Chin, L.S.

Current Medicinal Chemistry. (200) 15(1), 47-60.

Parkin-mediated K63-linked polyubiquitination: a signal for targeting misfolded proteins to the aggresome-autophagy pathway.

Olzmann, J.A., Chin, L.S.

Autophagy. (200) 4(1), 85-87.

Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding HDAC6.

Olzmann, J.A., Li, L., Chudaev, M., Perez, F.A., Palmiter, R. D., Chin, L.S.

Journal of Cell Biology. (2007) 178, 1025-1038.

PINK1 protects against oxidative stress-induced apoptosis by phosphorylating mitochondrial chaperone TRAP1/Hsp75.

Pridgeon, J.W.*, Olzmann, J.A.*, Chin, L.S., Li, L.

* These authors contributed equally.

PLOS Biology. (2007) 5, e172.

  – Featured in News and Views by Asa Abeliovich (Nature 448, 759-760)

  – Featured in Primer by Kroemer and Blomgren (PLoS Biol 5(7): e206)

Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking.

Kim, B.Y.*, Olzmann, J.A.*, Barsh, G.S., Chin, L.S., Li, L. 

* These authors contributed equally.

Molecular Biology of the Cell. (2007) 18, 1129-1142.

Selective enrichment of DJ-1 protein in primate striatal neuronal processes: Implications for Parkinson’s disease.

Olzmann, J.A., Bordelon, J.R., Muly, E.C., Rees, H.D., Levey, A.I., Li, L, Chin, L.S.

Journal of Comparative Neurology. (2007) 500, 585-599.

Oxidative damage of DJ-1 is linked to sporadic Parkinson's and Alzheimer's diseases.

Choi, J., Sullards, C. M., Olzmann, J. A., Rees, H. D., Weintraub, S. T., Bostwick, D. E., Gearing, M., Levey, A. I., Chin, L. S., Li, L.

Journal of Biological Chemistry. (2006) 281, 10816-24.

Uncoupling proteins prevent glucose-induced neuronal oxidative stress and programmed cell death.

Vincent, A. M., Olzmann, J. A., Brownlee, M., Sivitz, W. I., and Russell, J. W.

Diabetes. (2004) 53, 726-734.

Familial Parkinson's disease-associated L166P mutation disrupts DJ-1 protein folding and function.

Olzmann, J. A., Brown, K., Wilkinson, K. D., Rees, H. D., Huai, Q., Ke, H., Levey, A. I., Li, L., and Chin, L. S.

Journal of Biological Chemistry. (2004) 279, 8506-8515.

High glucose-induced oxidative stress and mitochondrial dysfunction in neurons.

Russell, J. W., Golovoy, D., Vincent, A. M., Mahendru, P., Olzmann, J.A., Mentzer, A., and Feldman, E. L.

FASEB Journal. (2002) 16, 1738-1748.